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LPS Induced TNF-a Production in Mice

After intraperitoneal administration, LPS, a constituent of the cell wall of Gram-negative bacteria, binds to a lipopolysaccharide binding protein (LBP) in blood. The LPS-LBP complex is a ligand for the CD14 receptor at the surface of monocytes and macrophages. The interaction of LPS-LBP with the receptor triggers the secretion of TNF-a. The anti-inflammatory activity of test compounds is evaluated 90 minutes after LPS administration (main and facultative read-outs).  

Species, strain, sex:
mouse, Balb/c, male
Number of animals /group:
Pharmacological control:
Routes of administration:
Treatment mode:
prophylactic, therapeutic
Duration of dosing:   
1 day or upon request

Main read-outs:
TNF-a concentration in serum

Facultative read outs:
concentration of other inflammatory  mediators in serum

LPS-induced TNF-a production in mice



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